10. _Saturated Aqueous Solution._

Weigh out

Basic fuchsin 1.5 grammes

and proceed as in preparing the corresponding solution of methylene-blue (_q. v._).

11. _Saturated Alcoholic Solution._

Weigh out

Basic fuchsin 3.5 grammes

and proceed as in preparing the corresponding solution of methylene-blue.

12. _Carbolic Fuchsin_ (Ziehl).

Weigh out

Basic fuchsin 1.0 gramme Carbolic acid 5.0 grammes

dissolve in

Distilled water 100.0 c.c.

and add

Absolute alcohol 10.0 c.c.

Filter.

CONTRAST STAINS.

~Eosin.~--There are several commercial varieties of eosin, which, from the bacteriological point of view, possess very different values. Gruebler lists four varieties, of which two only are useful for bacteriological work:

Eosin, aqueous yellowish.

Eosin, aqueous bluish.

13. _Eosin Aqueous Solution_ (Yellowish or Bluish Shade), 1 per cent.

Weigh out

Eosin, aqueous 1.0 gramme

dissolve in

Distilled water 100.0 c.c.

and add

Absolute alcohol 5.0 c.c.

Filter.

14. _Eosin Alcoholic Solution_, 0.5 per cent.

Weigh out

Eosin, alcoholic 0.5 gramme

and dissolve in

Alcohol (70 per cent.) 100.0 c.c.

Filter.

~Safranine.~--

15. _Aqueous Solution._

Weigh out.

Safranine 0.5 gramme

and dissolve in

Distilled water 100.0 c.c.

Filter.

~Neutral Red.~--

16. _Aqueous Solution._

Weigh out

Neutral red 1.0 gramme

and dissolve in

Distilled water 100.0 c.c.

Filter.

~Vesuvin (or Bismarck Brown).~--

17. _Saturated Aqueous Solution._